Anti-idiotypic and anti-anti-idiotypic antibodies as highly specific tools in epitope and active site studies on human interferon-γ
Molecular Immunology(1991)
摘要
Immunization of rabbits with F(ab')2 fragments of different monoclonal antibodies directed against human interferon-γ yielded antisera with anti-idiotypic characteristics. Isolation of the antiidiotypic fraction, resulting in a highly specific antiserum, allowed us to prove that out of six competing monoclonal antibodies directed against human interferon-γ, only two really recognize the same epitope. The other monoclonal antibodies compete on the basis of steric hindrance, which is not surprising, because of the large difference in Mr between interferon-γ and an immunoglobulin.
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关键词
HuIFN,Ig,MEM
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