Hepatitis C virus expressing flag-tagged envelope protein 2 has unaltered infectivity and density, is specifically neutralized by flag antibodies and can be purified by affinity chromatography

    Virology, Volume 409, Issue 2, 2011, Pages 148-155.

    Cited by: 7|Bibtex|Views1|Links
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    Keywords:
    hvr1moivaccinehypervariable region 1multiplicity of infectionMore(15+)

    Abstract:

    Hepatitis C virus (HCV) purification by ultracentrifugation is difficult because of the low and heterogeneous density of native and cultured viruses. It was recently shown that inserting flag tag into envelope protein 2 (E2) of HCV permitted virus purification by affinity chromatography. However, flag-tagged viruses had drastically altere...More

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