recA mutations reduce adherence and colonization by classical and El Tor strains of Vibrio cholerae.

Two recA mutants of Vibrio cholerae (classical and El Tor biotypes) were constructed by disruption of the wild-type recA gene with mutated recA sequences of V. cholerae cloned in the suicide vector pGP704. Mutants defective in the recA gene were compared with their respective RecA(+) parent strains with regard to their adherence to isolated rabbit intestine and Maryland, School of colonization of intestine of infant mice. The recA mutation in V. cholerae was Medicine, Baltimore, found to diminish adherence and markedly affected colonization.