Urea-Formaldehyde Resin Microspheres As A New Packing Material For Liquid Chromatography: Ligand Exchange

A new ligand exchange chromatography (LEC) packing material consisting of L-proline modified urea-formaldehyde resin microspheres was prepared and tested. The experiments show that the material has a good enantioselectivity for the chiral separation of amino acid racemates. Solvent acetonitrile and high column temperature are favorable for enhancing the selectivity. There is an optimal ammonia concentration in the eluent for the LEC separation. Among the 17 DL-amino acids separated, 16 have the D-eluted ahead of the L-enantiomers, except for proline.