High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics Using a FLUOstar Omega

6 cells per millilitre and then diluted one hundred fold into fresh YPD broth. TTO and AMB were then prepared in YPD containing 0.05% v/v Tween 80 at 0.01, 0.05, 0.1 and 0.5% for TTO and 0.0002, 0.0001, 0.00005 and 0.000025% w/v for AMB. Yeast cells were diluted into each TTO suspension to a final concentration of 1×10 4 cells/mL, then 200 µL volumes arrayed in quadruplicate into adjacent columns of a 96-well microtitre plate. A negative control without any reagent was included. The plate was placed inside the FLUOstar Omega with the incubator preset to 37°C. The absorbance mode was set to read at 550 nm with orbital shaking for 30 seconds prior to each read. This was programmed to take forty eight individual measurements over a 24 hour period.