Effect Of Astragalus Complanatus Flavonoid On Anti-Liver Fibrosis In Rats
World journal of gastroenterology : WJG(2005)
摘要
AIM: To observe the anti-liver fibrosis effect of Astragalus complanatus flavonoids (ACF) in rats.METHODS: The liver fibrosis model in rats was established by injecting interperitoneally 0.2 mL/100 g 0.5% dimethylnitrosamine, thrice a week. Meanwhile, the rats were administered ACF (30, 60, 120 mg/kg) or colchicine (0.1 mg/kg) once a day for 1 mo. Serum N-propeptide of type I procollagen (PINP) and type III procollagen (PIIINP) was measured using ELISA. Malondialdehyde (MDA) and superoxide dismutase (SOD) in hepatic tissue were evaluated. Matrix metal protease-1 (MMP-1) mRNA expression was assayed by RT-PCR and the protein expression of tissue inhibitor of metal protease-1 (TIMP-1) was analyzed by immunohistochemistry.RESULTS: In the ACF groups, SOD activity increased and MDA content decreased in comparison to the liver fibrosis model group. The serum PINP and PIIINP contents in ACF-2 and -3 group decreased compared to those in model group. In ACF-2 and -3 group, the expression of MMP-1 mRNA increased significantly and the protein expression of TIMP-1 decreased compared to that in model group.CONCLUSION: The antifibrotic mechanisms of ACF are associated with its influence on lipid peroxidation and collagen synthesis and degradation. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
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关键词
Astragalus complanatus,Liver fibrosis,N-propeptide of type I procollagen,N-propeptide of type III procollagen,Malondialdehyde,Superoxide dismutase,Matrix metal protease-1,Tissue inhibitor of metal protease-1
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