Double chip protein arrays using recombinant single-chain Fv antibody fragments.
PROTEOMICS(2004)
摘要
Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be pre-screened in order to reduce false positives. In contrast, we use a second chip surface for the local application of detection antibodies, thereby efficiently eliminating antibody cross-reactions. Here, we illustrate the potential advantages of using single-chain Fv fragments rather than mAbs as capture and detection molecules with this double chip technology.
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关键词
multiplexing,protein array,recombinant antibody,sandwich immunoassay
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