Clonning and Expression of RgPR-10 Gene from Rehmannia glutinosa

In order to investigate the storage root development mechanism of Rehmannia glutinosa,SSH(suppression subtractive hybridization) was performed to isolate storage root development related genes.One high copy RgPR-10 gene(GenBank,Accession No.EU526395) with complete open reading frame which encoded 154 amino acids was isolated from the SSH library.The RgPR-10 gene of Rehmannia glutinosa shared low identity with other species.Northern blotting analysis showed that RgPR-10 was expressed mainly in root and stem and accumulated in storage root at high level.In order to further investigate its functions in Rehmannia glutinosa,the RgPR-10 gene was expressed in E.coli BL21 and purified by gel purification method.The purity of the purified protein is relative high and can be used for further study.