Fertilization and the cytoskeleton in the red alga Bostrychia moritziana (Rhodomelaceae, Rhodophyta)

Time-lapse videomicroscopy Was used to observe the effects of various cytoskeletal inhibitors on three important fertilization events in Bostrychia moriziana: spermatial mitosis, gamete fusion (formation of a fertilization pore) and nuclear migration along the trichogyne. The microtubule inhibitor oryzalin disrupted spermatial mitosis but had no other effect on fertilization. The actin inhibitors. jasplakinolide, cytochalasin B. latrunculin A and B and mycalolide B inhibited gamete fusion while BDM, a myosin-disrupting drug. inhibited all three major fertilization events, FL-Phallacidin was used to stain actin filaments in spermatia and trichogynes awhile microtubules were labelled with antibodies at appropriate stages of fertilization. Microtubules were only evident during spermatial nuclear division. Actin filaments were present in both trichogynes and spermatia throughout fertilization they formed a discrete ring around the fertilization pore and ensheathed male nuclei as the latter migrated into and along the trichogyne. These results Suggest that the actin/myosin system plays a role in the events of fertilization.