Simultaneous identification of multiple receptors of natural product using an optimized cDNA phage display cloning.

Qing-Li He,Hui Jiang,Feng Zhang,Hai-Bao Chen,Gong-Li Tang

Bioorganic & Medicinal Chemistry Letters（2008）

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摘要

An optimized cDNA phage display cloning procedure is reported to simultaneously isolate two protein targets of cyclosporine A dependent on the binding affinity rather than the relative abundance in cells.

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关键词

Display cloning,Cyclosporine A,Cyclophilin A,Cyclophilin B

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