Estimation of protein molecular weights by high performance molecular-sieve chromatography on agarose colums in sodium dodecyl sulphate solution

Calibration curves showing the linear relationship between — log KD and (molecular weight) 23 are presented for proteins subjected to molecular-sieve chromatography in 1 0.1% sodium dodecyl sulphate solution on crosslinked gels of different agarose concentrations (5, 9, 12 and 20%). These gels permit separation and estimation of molecular weights of proteins in the range 5000 to about 400 000, 130 000, 130 000, and 100 000, respectively. Separations of model proteins, heavy and light chains of γ-globulin, and cyanogen bromide fragments of cellulase are shown.