Efficient plant regeneration from cotyledons of black gram ( Vigna mungo (L.) Hepper)

An efficient and rapid regeneration protocol was developed from cotyledons of black gram (Vigna mungo (L.) Hepper) variety T-9. Murashige and Skoog's (MS) medium supplemented with Gamborg B 5 vitamins containing BAP @ 4.0 mg/L was most effective in producing regenerative calli. The cultures expressed maximum plant regeneration potential with 12 shoots per calli on regeneration media with no exogenous amino acid or plant growth regulator supplementation. Green and robust shoots thus devel- oped were successfully rooted within 15 d on 1/3 MS medium. Over 90% of rooted plants grew well and produced seeds nor- mally when transferred to green house.