Effect of nitric oxide on the lateral root's generation of mung bean.

【Objective】 Effect of nitric oxide(NO)on lateral rooting of mung bean was investigated.【Method】 With mung bean as study material,the radicles were treated with sodicem nitroprusside(SNP),c-PTIO of NO specific scavenger and NOS inhibitor L-NAME alone or plus IBA or NAA to analyze the effect of endogenous NO on lateral rooting of mung bean.【Result】 The results showed that SNP could significantly enhance the lateral rooting and the effects of IBA or NAA in stimulating rooting,50 μmol/L SNP for 24 h being the best,average lateral roots per plant 18.c-PTIO of NO specific scavenger,or NOS inhibitor L-NAME treated alone or plus IBA or NAA resulted in an inhibitory effect.【Conclusion】 It's indicated that endogenous NO appears to play a key role in the generation and development of lateral roots.