EVALUATION OF ANALGESIC AND ANTIMICROBIAL ACTIVITY OF DIFFERENT FRACTIONS OF CRUDE METHANOL EXTRACT OF TINOSPORA CRISPA STEM.

Tinospora crispa, a plant belonging to the family of Menispermaceae is a well-known traditional medicinal plant used in Bangladesh. This study has carried out to evaluate the analgesic (by acetic acid induced writhing test) activities by using Swiss albino mice as a test animal. At a dose of 400 mg/kg body weight, the crude methanol extract and its other fractions of stem significantly (p<0.05) produced inhibition of writhing compared to the Standard (Diclofenac Sodium). Among all the fractions, Petroleum ether soluble fraction showed most significant analgesic activity (51.94%) compare with standard (65.12%). In addition the crude extract and its fraction showed no significant antimicrobial activities against five gram-positive, eight gram-negative bacteria and three fungi compare to the standard (kanamycin).