Inheritance of RAPD markers in an interspecific F1 hybrid of grape between Vitis quinquangularis and V. vinifera

Inheritance of randomly amplified polymorphic DNA (RAPD) markers in an interspecific F1 hybrid between Vitis quinquangularis (‘83-4-96’) and V. vinifera (‘Muscat Rose’) were studied. A preliminary screen of the F1 plants and the parents used as the interspecific cross parent was conducted with 280 RAPD primers; of these, 39 primers were polymorphic and reproducible and produced 234 scorable loci. Genomic DNA from 60 individuals of the F1 population was then amplified with these primers; 83.4% of the loci segregated as expected in a Mendelian fashion. According to the segregation patterns, RAPD markers were grouped into three types: (1) normal Mendelian inheritance with segregation ratio of nearly 1:1, 3:1 or 1:0 (non-segregation); (2) deviation from Mendelian segregation ratios; (3) abnormal segregation in low frequency, including non-parental markers which were present in F1 progenies but absent in both parents. RAPD markers presenting in both parents were the major source of non-segregation markers; markers presenting in only one parent mainly showed Mendelian segregation. There were five non-parental markers. Markers showing 1:1 and 3:1 segregation were used for constructing an RAPD molecular linkage map. The results demonstrated that RAPD markers could be used to study the genomes of the wild grapes native to China.