Site of Analgesic Effect of Total Saponin from Thladiantha dubia Root

Objective:To invastigate the site of analgesic effect of the total saponin from Thladiantha dubia root(TSRT).Method: Kunming mice 120 were devided into formalin test and improved Randall-Selitto method grougs,60 in each groug.Sixty mices were divided into TSRT 240,120,60 mg·kg-1grougs,Asp 200 mg·kg-1groug,Mor 5 mg·kg-1,grpop and NS group.The Mor group was given by sc,the others were given by ig.The site of analgesic effect of TSRT respectively was analysed by formalin test and mend Randall-Selitto method in mice.Result:In formalin test in mice,TSRT of 120,60 mg·kg-1 significantly lowered the pain score of the second phase(P0.05 vs NS group),but there is no effect on the pain score of the first phase(P0.05 vs NS group),TSRT of 240 mg·kg-1 lowered both the pain scores of the first phase and second phase(P0.05-0.01 vs NS group).In imoroved Randall-Selitto method in mice,TSRT markedly increased the pain threshold value on inflammation foot(P0.01 vs NS group),but pain threshold value in normal foot did not obviously elevated(P0.05 vs NS group).Conclusion: The site of analgesic effect of TSRT is mainly located in the periphery.