The effect of miRNA-214 on cell cycle and apoptosis of gastric cancer cell lines BGC823 and MKN45

Objective To identify the effects of miRNA-214 on cell cycle and apoptosis of gastric cancer cells,and its influence on PTEN protein level. Methods miRNA-214 in poorly differentiated human gastric cancer cell line BGC823,MKN45 and normal gastric mucosal cell line GES-1 was detected by real-time PCR. Antisense-miRNA-214 oligonucleotides(miRNA-214 inhibitor) were transfected transiently into gastric cancer cell lines to down-regulate the expression of miRNA-214. The cell cycle and apoptosis rate were studied by flow cytometry. PTEN,the target gene of miRNA-214,was detected by immunofluorescence. Results The miRNA-214 was upregulated in gastric cancer cell BGC823 and MKN45 (P0.05) compared with normal gastric mucosal cell line GES-1. The transfection of miRNA-214 inhibitor downregulated miRNA-214 expression in BGC823 (P0.05). G1-phase cells were increased in BGC823 and MKN45,[(60.20±3.38)% vs. (49.33±7.99)% (P0.05);(69.90±0.28) vs. (54.85±0.64)% (P0.05) respectively] compared with the control groups. But the alteration of apoptotic rate was not significant(P0.1). The immunofluorescence result showed that PTEN was upregulated after transfection in both cell lines. Conclusions (1)miRNA-214 is upregulated in human gastric cancer cell BGC823 and MKN45. (2)miRNA-214 could reduce the G1-phase cells and increase S-phase cells in BGC823 and MKN45,and the downregulation of PTEN may be one of the mechanisms. (3)miRNA-214 has no effect on apoptosis of BGC823 and MKN45.