Experimental thermal lesions induce beta-thromboglobulin release from activated platelets

Time courses of beta-thromboglobulin release, and protein extravasation after thermal inflammation of human skin was compared to neurogenic inflammation induced by histamine iontophoresis. Beta-TG and protein levels were sampled by intradermal microdialysis. Three microdialysis membranes were inserted at each stimulation site. The collected samples were measured photometrically for protein by the Coomassie blue method and for beta-TG by ELISA. Heat stimuli of 40°C and 47°C, and histamine iontophoresis were inflicted directly above the membranes. In vitro recovery rates of beta-TG and bovine albumin were measured using a latex chamber. Recovery rates at a continuous flow rate of 3μl/min for bovine albumin ranged between 20% and 35%; those for beta-TG ranged between 14% and 17%.