Immunophenotyping of murine peritoneal macrophages and lymphocytes by flow cytometry

Peritoneal cells include mainly macrophages and both T and B lymphocytes. We describe a simple procedure to analyze by flow cytometry the antigens expressed on macrophage and/or lymphocyte membranes. F4/80 and CR3 macrophage antigens were detected with anti-F4/80 and anti-Mac-1 monoclonal antibodies (mAb) respectively, CD4 and THY1.2 T lymphocyte antigens with GK1.5 and J1j mAbs respectively, B CD5 lymphocytes were identified with LY1.2 mAb and FcγRII/FcγRIII were detected with 2.4G2 mAb.