Levels of expression of heat shock proteins 60, 70, and 90α in colorectal cancer according to Western blot and their relationship to clinical pathology.

Objective To study levels of expression of the proteins HSP60,70,and 90α in colorectal cancer and to analyze the relationship between the expression of HSP60,70,and 90α and the pathology of and prognosis for colorectal cancer.Methods Collection of colorectal cancer samples: fresh colorectal cancer tissues and adjacent normal tissues 5 cm from the tumorous tissue were collected from 49 patients.This was done to detect the level of expression of HSP60,HSP70,and HSP90α proteins in colorectal cancer using Western blot.Results Western blot revealed that the expression of HSP60,HSP70,and HSP90α in tumor tissues compared to that in adjacent non-tumorous tissues was as follows: HSP60/β-actin(1.409±0.899 versus 0.434±0.122),HSP70/β-actin(0.955±0.247 versus 0.405±0.125),and HSP90α/β-actin(0.873±0.199 versus 0.364±0.108).The relative level of expression increased significantly in tumor tissues and adjacent non-tumorous tissues for all the three HSP family genes.Conclusion Results showed that HSP60,HSP70,and HSP90α proteins in colorectal cancer were significantly up-regulated in tumor tissues compared to their expression in adjacent non-tumorous tissue.The high expression of these proteins in colorectal cancer tissues may indicate that they play an important role in the pathogenesis of colorectal cancer.