The presence of neuropeptides in GABAergic and cholinergic rat cerebrocortical synaptosome sub-populations

GABAergic and cholinergic synaptosomes from rat cerebral cortex were isolated by a magnetic immunoaffinity technique, i.e. immunomagnetophoresis. These subpopulations were extracted and subjected to radioimmunoassay for four neuropeptides: Neuropeptide Y (NPY); vasoactive intestinal peptide (VIP); substance P (SP); and somatostatin (SRIF). In each of the sub-populations three of the four peptides were enriched in the sorted fraction compared with the mother fraction with respect to the cytosolic marker lactate dehydrogenase (LDH). In the GABAergic sub-population the order was SP>SRIF>NPY≥VIP whilst in the cholinergic sub-population they were enriched in the order VIP≥NPY>SP>SRIF. The presence of NPY has not previously been reported in cortical cholinergic neurons.