Immunoprecipitation of the in vitro translation product of rabbit muscle protein phosphatase C-I mRNA
Biochemical and Biophysical Research Communications(1988)
摘要
Rabbit muscle polyA + mRNA was translated in vitro using a rabbit reticulocyte lysate system in the presence of [ 35 S]methionine. A mouse monoclonal antibody to the catalytic subunit of rabbit muscle phosphorylase phosphatase (“phosphatase C-I”) was used to immunoprecipitate the products which were then analyzed by SDS-PAGE and autoradiography. These studies showed that the major product of the phosphatase mRNA is a single ca . 36 kDa polypeptide. These findings are significant in view of suggestions that the catalytic subunit is derived from a larger precursor, and in view of the molecular cloning of two cDNAs for the phosphatase, which encode polypeptides of 35.4 kDa and 37.5 kDa, respectively.
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关键词
protein phosphatase
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