One new method of nucleic acid amplification—Loop-mediated isothermal amplification of DNA
Virologica Sinica(2008)
摘要
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.
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关键词
Nucleic acid amplification,Loop-mediated isothermal amplification (LAMP),Application
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