Simple And Rapid Method Determination For Metformin In Human Plasma Using High Performance Liquid Chromatography Tandem Mass Spectrometry: Application To Pharmacokinetic Studies
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences(2007)
摘要
A rapid and simple method for quantitation of metformin (MET) in human plasma by HPLC-MS/MS was developed and validated. The sample preparation consists of plasma deproteinization using acetonitrile. The mobile phase consisted of water-acetonitrile and formic acid (55/45/0.048, v/v/%) and the run time was 3 min. A pursuit C-18 (100 mm x 2.0 mm i.d., 3 mu m) column connected to a guard column MS-pursuit (0.20 mm x 0.20 mm i.d., 5 mu m) was used. The range of the calibration curve was from 20 to 5000 ng/mL, the limit of quantitation being 20 ng/mL. The detection was performed on a mass spectrometer (ESI+), using metoprolol as internal standard. The calibration curves have r(2) values of 0.995 (CV = 0.24%, n = 10). The accuracy and precision were between 90.74 and 106.7% and coefficients of variations (CV) of 1.10 and 4.35%, respectively. The method was applied to determine the pharmacokinetic parameters: C-max (1667.25 ng/mL) and T-max (3.89 h). (c) 2007 Elsevier B.V. All rights reserved.
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关键词
metformin,liquid chromatography,mass spectrometry,plasma,pharmacokinetic
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