Chitosan/Carboxymethyl Cellulose Polyelectrolyte Complex Scaffolds for Pulp Cells Regeneration
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS(2007)
摘要
Novel polyelectrolyte complex (PEC) chitosan/carboxymethyl cellulose (CMC) scaffolds are prepared using a freeze-dry process. The microstructure is characterized by SEM and the average pore diameters and internal porosities are calculated. The morphology and distribution of pulp cells on these three-dimensional scaffolds are investigated by SEM and confocal laser scanning microscopy (CLSM). The expression of osteonectin (ON) and dentin sialophosphoprotein (DSPP) is detected by reverse transcription polymerase chain reaction (RT-PCR). The scaffolds are then implanted subcutaneously into BALB/c mice. The addition of CMC to chitosan decreases the pore diameter and increases internal porosity. The pulp cells co-cultured in these PEC scaffolds show improved adhesion, spreading, cell capacity, and three-dimensional configurations compared to pure chitosan scaffolds. Based on the data obtained, the chitosan/CMC complex provides an improved scaffold material for pulp tissue engineering.
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关键词
polyelectrolyte complexes,polyelectrolyte complex scaffolds,chitosan,chitosan scaffolds,carboxymethyl cellulose,tissue engineering,pulp tissue regeneration
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