Vectors with restriction-site banks III. Escherichia coli-Saccharomyces cerevisiae shuttle vectors

M Heusterspreute,J Oberto,H T Vinh,J Davison

Gene（1985）

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摘要

The bank of unique restriction sites present in plasmid pJRD158 has been incorporated into new vectors carrying selective markers and replicons derived from commonly used Escherichia coli-Saccharomyces cerievisiae shuttle vectors pJDB207 and YRp7. The new vectors pMH158 and pJ0158 have 21 and 23 unique restriction sites, respectively, and their complete DNA sequences are known.

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关键词

Recombinant DNA,LEU2,TRP1,ARS 1,2λ plasmid,yeast cloning,restriction map,DNA sequence

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