Pyridoxylated Polymerized Hemoglobin Solution Processing
Applied Biochemistry and Biotechnology(1987)
摘要
Glutaraldehyde hemoglobin polymerization gives too many high polymers, resulting in a too viscous solution. We describe here
an alternate method leading to superior results, as compared to the classical one. This method includes a molecular fractionation
step using a tangential flow ultrafiltration that secondarily lowers the unpolymerized tetramer’s content of a mildly polymerized,
pyridoxylated hemoglobin solution (Pyr-Poly Hb). This leads to an adequately polymerized product with a lesser high polymer
content, implying a lower viscosity. We thus obtain a pyridoxylated, polymerized molecular fractionated solution presenting
suitable features as a blood substitute: A 7.5 g% hemoglobin 2 g% albumin solution had a 16% unpolymerized tetramer’s ratio,
a 1.8 mPas viscosity, a P50 of 2.8 kPa, a Hill coefficient of 2.1, a binding coefficient of 1.3 mL/g, a colloid osmotic pressure of 2.4 kPa, and a methemoglobin
concentration of 3% Male Sprague-Dawley rats undergoing an isovolumic blood exchange with this Pyr-Poly Hb solution, down
to a 2% hematocrit, present a mean survival time of 20 h.
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关键词
colloid osmotic pressure
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