Complete H-1 And C-13 Nmr Assignments Of Three New Polyhydroxylated Sterols From The South China Sea Gorgonian Subergorgia Suberosa

Three new polyhydroxylated sterols, 3 beta,6 alpha,11,20 beta,24-pentahydroxy- 9,11-seco-5 alpha-24-ethylcholest-7,28-diene-9-one (1), 3-(1',2'-ethandiol)-24- methylcholest-8(9),22E-diene-3 beta,5 alpha,6 alpha,7 alpha,11 alpha-pentaol (2), 24-methylcholest-7,22 E-diene-3 beta,5 alpha,6 beta,25-tetraol (3) together with five known sterols, were isolated from the EtOH/CH2Cl2 extract of the South China Sea gorgonian Subergorgia suberosa. The complete assignments of the H-1 and C-13 NMR chemical shifts for these new compounds were achieved by means of 1D and 2D NMR techniques, including HSQC, HMBC, H-1-H-1 COSY, and NOESY spectra. Copyright (c) 2007 John Wiley & Sons, Ltd.