Comparison of motility and membrane integrity to assess rat sperm viability
REPRODUCTIVE TOXICOLOGY(1998)
摘要
Rat sperm motility and membrane integrity were compared as endpoints for viability, Sperm motility was measured by computer-assisted semen analysis (CASA), whereas membrane integrity was assessed by flow cytometric analysis of sperm stained with two nucleic acid stains, SYBR-14 and propidium iodide, The two techniques were compared in experiments that examined sperm viability over time and by analysis of known mixtures of control and freeze/thaw-killed sperm, Results from the two approaches were quantitatively very similar, Sperm from rats treated with dibromoacetic acid (600 or 1200 mg/kg) or alpha-chlorhyrin (100 mg/kg) were also analyzed. Neither technique detected a treatment-related effect with dibromoacetic acid, CASA identified a significant decrease in sperm motility in alpha-chlorhyrin-treated rats, whereas flow cytometric analysis did not find a measureable change in sperm membrane integrity, Because decreases in sperm motility would be expected to directly affect fertility, CASA may be a more robust endpoint for risk assessment in reproductive toxicology studies than flow cytometric analysis of membrane integrity. (C) 1998 Elsevier Science Inc.
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关键词
sperm,computer-assisted semen analysis (CASA),viability,flow cytometry,propidium iodide (PI),SYBR-14,dibromoacetic acid (DBAA),alpha-chlorhyrin (ACH)
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