A reversible effect of low carbon monoxide concentrations on the EPR spectra of the periplasmic hydrogenase from Desulfovibriovulgaris

Daulat S. Patil,Melvin H. Czechowski,Boi H. Huynh,Jean LeGall,Harry D. Peck,Daniel V. DerVartanian

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS（1986）

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摘要

The effect of low concentrations of CO (0.93 – 5.58 μM) on the EPR spectrum of the periplasmic non-heme iron hydrogenase from D. vulgaris has been investigated. The “g=2.06” EPR signal is maximally induced (0.94 spin/molecule) at 46.5 μM CO and partial induction of the EPR signal could be observed at 0.93 μM CO. This effect is reversed by removal of the CO or irradiation of the hydrogenase with white light.

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关键词

2,6-dichlorophenol-indophenol,dcpip,electron paramagnetic resonance,epr,electron spin resonance,iron,spectrum,magnetic resonance,carbon monoxide,enzyme,sulfate reducing bacteria

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