RFLP of analyses of an intergenic spacer region of chloroplast DNA in some wild wheat species

Several studies are being made to get high productive wheats throughout the world because they provide the most of human energy and protein needs. In this study, 11 wheat species of Triticum and Aegilops were investigated. One of the intergenic regions of cpDNA was studied. This region was amplified with PCR and digested with 6 restriction endonucleases (Hpa II, Alu I, Hinc II, Ava III, Nde I and Hae III). According to results, amplified products can be divided into the three groups which are Group 1 (similar to 2000 bp), Group 2 (similar to 1000 bp) and Group 3 (similar to 700 bp). Group 1 contains Ae. neglecta Req. ex Bertol. (syn.: Ae. triaristata), Ae. triuncialis, Ae. mutica Boiss. and T. monococcum L. var. monococcum. Group 2 contains T. aestivum L., Ae. biuncialis Vis., T. turgidum L. (Syn.: T. turgidum var. turgidum) and T. carthlicum Nevski. Group 3 contains Ae. tauschii Cosson. subsp. tauschii and Ae. cylindrica Host. Data obtained from the restriction enzyme digestions were evaluated by using numerical taxonomy system and a phenogram was constructed using SAHN clustering unweighted pair group method using arithmetic averages.