A longitudinal cohort study in calves evaluated for rotavirus infections from 1 to 12 months of age by sequential serological assays
Archives of Virology(2009)
摘要
Using an immunocytochemical staining assay involving six different recombinant baculoviruses with each expressing one of the
major bovine rotavirus VP7 (G6, G8 and G10) and VP4 (P6[1], P7[5] and P8[11]) serotypes, we analyzed IgG antibody responses
to individual proteins in archival serum samples collected from 31 calves monthly from 1 to 12 months of age during 1974–1975
in Higley, Arizona. Seroresponses to VP7 and VP4, as determined by a fourfold or greater antibody response, were not always
elicited concurrently following infection: in some calves, (1) seroresponses to VP7 were detected earlier than to VP4 or vice
versa; and (2) a subsequent second seroresponse was detected for VP7 or VP4 only. In addition, a second infection was more
likely to be caused by different G and/or P types. Analyses of serum samples showed that the most frequent G–P combination
was G8P6[1], followed by G8P7[5], G8P8[11] and G6P6[1].
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关键词
Complement Fixation Test, Recombinant Baculoviruses, Neonatal Calf Diarrhea, Archival Serum Sample, NCDV Strain
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