Variable toxicity of dyes used in micropuncture studies

This study evaluated the potential toxicity of Fast green and Lissamine green, the two dyes commonly used for visualizing tubular fluid flow in micropuncture studies. In both the in vitro perfused kidney and cultured tubular epithelial cells, Fast green exhibited a profound, dose-dependent toxicity. In contrast, Lissamine green was non-toxic. In conclusion, we recommend that Fast green not be used in micropuncture studies.