Detection of aminoglycosides modifying enzyme genes of pseudomonas aeruginosa

OBJECTIVE To investigate the situation of aminoglycosides modifying enzyme encoding(AMEs)genes of Pseudomonas aeruginosa.METHODS The aminoglycosides modifying enzymes(AMEs) genes of 59 isolated strains of PAE were determined by polymerase chain reaction(PCR).RESULTS Totally 35 strains of PAE containing aac(6′)-Ⅱ+ant(3″)-Ⅰ+ant(2″)-Ⅰof AMEs genes were detected;The resistance phenotypes of AMEs genes to gentamicin and amikacin had the higher positive predictive value.CONCLUSION The aminoglycosides modifying enzymes genes detected in this study show higher coincidence rates with gentamicin and amikacin-resistant PAE.