Detection and analysis of group diarrhea event from a foreign ship

Objective To collect the samples from infected sailors and develop a inspection at laboratory for the pathogeny of the group diarrhea event.To take some measures to protect the healthy sailors of the infected foreign ship and to control the spread of the epidemic.Method Considering epidemiological analysis,the rectal swabs,stools were collected and the pathogens as Salmonella,Vibrio cholera,Vibrio parahemolyticus and Escherichia coli O157:H7 were detected with real-time fluorescent PCR.The methods of biochemical analysis and serological typing were also used to identify the separated strains.Result The result of real-time fluorescent PCR showed that 13 samples were positive of Salmonella nucleic acid and there were not any other pathogens were detected.The positive separated strains were identified to be Salmonella II with biochemical and serological analysis.Conclusion The pathogeny of the group diarrhea event is the infection of Salmonella II Foods and drinking water are presumbed to be the most possible pollutant source,but we have not found the pathogen of Salmonella from either all kinds of foods,drinks or environmental samples.