Bead transfection of adherent cells
Molecular Biotechnology(1996)
摘要
Bead transfection is a simple, rapid, efficient, and cost-effective method of gene transfer into adherent mammalian cells.
It involves a brief incubation of the cells with glass beads in a solution containing the DNA to be transferred. We have optimized
this technique using COS-7 (an SV40 transformed monkey kidney cell line) and a transient expression assay for chloramphenicol
acetyl transferase (CAT). Stable transfection efficiency assessed using the selectable marker gene neomycin phosphotransferase
(NEOR) was 27% in COS-7 cells. As this technique delivers high transfection efficiency with little manipulation of the exogenous
DNA and does not require the use of any viral sequences, it may be a useful alternative method of gene delivery in the development
of gene therapy protocols.
更多查看译文
关键词
cost effectiveness,gene transfer,gene delivery,cell line
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要