In vitro effects of buyang huanwu decoction and its ingredients on inhibiting the specific binding of 3H-platelet activating factor to its receptor in rabbits

Methods This experiment was carried out in the Laboratory of Nuclear Medicine, Guangzhou University of Traditional Chinese Medicine between September and December 2004. The seven influencing factors were selected: such as Shenghuangqi, Dangguiwei, Chishao, Dilong, Taoren, Honghua, Chuanxiong . Each factor was divided into two levels, selected or not selected. The tests were arranged according to L 8 (2 7 ) orthogonal test table. The specific binding of 3 H-PAF to its receptors in rabbits was measured by radioligand binding assay. The inhibitory rate of the specific binding was used as an assessing index. The inhibitory action of and on 3 H-PAF to PAFR binding was analyzed and compared in vitro . The inhibitory action of each ingredient in the prescription BHD on 3 H-PAF to PAFR binding was investigated and compared in vitro by direct analysis and analysis of variance of orthogonal test. Main outcome measures Effect of 8 prescriptions for L 8 (2 7 ) orthogonal test table on the specific binding inhibition rate of 3 H-PAF and PAFR. Results According to results of variance analysis of orthogonal test, the inhibitory action of each ingredient in the prescription BHD on 3 H-PAF to PAFR binding from the highest to the lowest was in turn Honghua, Shenghuangqi, Taoren, Dilong, Dangguiwei, Chuanxiong, Chishao. Honghua, Shenghuangqi, Taoren, Dilong, Danguiwei were major influence factors to 3 H-PAF to PAFR in rabbits ( F = 187.829,144.446,59.521,5.018,4.265, P < 0.05–0.01), but Chuanxiong and Chishao had not obviously inhibitory effect. The specific binding inhibition rate of prescriptions (except Shenghuangqi ) was obviously higher than that of one of prescriptions ( Shenghuangqi included). Conclusion The results of orthogonal test show that Honghua, Shenghuangqi, Taoren, Dilong, Dangguiwei are major influencing factors to inhibit binding of 3 H-PAF to PAFR in rabbits, among which, Honghua is the strongest in ingredients of prescription BHD. The results also reveal that Shenghuangqi is able to weaken the inhibitory effect and to prevent the strong inhibitory effect of blood-activating drugs in BHD. Key Words buyang huangwu decoction radioligand binding assay platelet activating factor orthogonal test