Gene Identification By Chromosomal In-Situ Hybridization, Microdissection And Polymerase Chain Reaction Amplification

This paper describes a theoretical method by which candidate genes for inherited diseases can be identified by polymerase chain reaction amplification and subsequent cloning of organ-specific complementary deoxyribonucleic acid libraries hybridized in situ to, and subsequently dissected from, the cognate chromosome. A specific application of this technique to isolate genes related to genetic haemochromatosis is outlined.