Optimization of a radiolabel DNA-binding assay in cultured mammalian cells
Mutation Research/Genetic Toxicology and Environmental Mutagenesis(2011)
摘要
An improved protocol for the radiolabel DNA-binding assay, which gives a high yield of highly pure DNA has been developed by use of mouse lymphoma cells. The critical difference from previously published methods is the use of enzymatic degradation of proteins in the later DNA purification steps rather than during the homogenisation procedure.
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关键词
DNA binding,DNA purification,Protein binding,Protein purification,Benzo[a]pyrene,Doxorubicin,Dopamine,Diazepam
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