ChemInform Abstract: Synthesis of a New Fluorogenic Substrate for the Continuous Assay of Mammalian Phosphoinositide-Specific Phospholipase C.

Aleksey V. Rukavishnikov,Tatiana O. Zaikova,G. Bruce Birrell,John F. W. Keana,O. Hyes Griffith

ChemInform（2010）

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摘要

The synthesis of a fluorogenic substrate for mammalian phosphoinositide-specific phospholipase C is described. The substrate, based on the widely used fluorescein molecule, is a water-soluble substrate analog of phosphatidylinositol-4-phosphate. The fluorogenic substrate 2 is shown to be a sensitive substrate for human PI-PLC-delta1 in a continuous assay.

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new fluorogenic substrate,cheminform abstract,phosphoinositide-specific

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