Two active forms of RD-114 virus DNA polymerase in infected cells

Two forms of DNA polymerase are present in RD-114-infected human, dog, and mink cells, but are not detectable in uninfected cells. The two enzymes are indistinguishable catalytically and immunologically, but differ with respect to molecular weight and elution position from (dT)12–18-cellulose and phosphocellulose. The large enzyme (∼ 95,000 daltons) is found in the infected cells, but not the virions produced by these cells. The virions contain only the smaller enzyme (∼ 70,000 daltons). The larger form may represent a mammalian viral equivalent to the β subunit of avian RNA tumor virus DNA polymerase.