A continuous assay of acetate kinase activity: Measurement of inorganic phosphate release generated by hydroxylaminolysis of acetyl phosphate
Bioorganic Chemistry(2008)
摘要
A novel continuous assay for acetate kinase has been developed that utilizes purine nucleoside phosphorylase-based measurement of the inorganic phosphate generated by hydroxylaminolysis of one of the products of the acetate-kinase reaction, acetyl phosphate.
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关键词
Acetyl phosphate,Acetate kinase,ASKHA phosphotransferase,Purine nucleoside phosphorylase,MESG
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