Isolation and identification insecticidal activity franctions from petroleum ether partitioned extract in Jatropha curcas L. branches and leaves

To make clear insecticidal activity components from Jatropha curcas L.branch leaves.Using alcohol crude extract of petroleum ether partitioned extract from Jatropha curcas L.branch leaves as an experimental material in our investigation,and then silica gel chromatography,combined with PTLC preparation.At same time,the each stage of separated products was carried out by tracking activity,analysis by GC-MS,structure elucidation the main compositions of exhibited active components.The results show that the concentration of colony AJ1 reached 8 mg/mL,Myzus persicae(Sulzer)and Pieris rapae L.both corrected mortality exceeded 70% in 24 h(72 h),showed no significant differences with imidacloprid(70%),showed that toxic activity was very high.The colony AJ1 analysis using GC-MS,the results showed,nine compounds with maximum contents were identification,total amount up to 96.18%,the first five of maximum contents were 2-Propanol,1-(allyloxy),4-Quinolnol,4-ethyldecahydro-1,2-dimethyl-,4-(allyloxy)-2-methyl-2-pentanol,2,4-Dimethyldecane and 1-(hexyloxy)octane.The total amount of five main constituents reached 83.81%.