Site-specific labeling of the ribosome for single-molecule spectroscopy

    Nucleic acids research, Volume 33, Issue 1, 2005, Pages 182-189.

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    Keywords:
    fluorescence spectroscopyribosomal rnamutationprotein synthesiskineticsMore(4+)

    Abstract:

    Single-molecule fluorescence spectroscopy can reveal mechanistic and kinetic details that may not be observed in static structural and bulk biochemical studies of protein synthesis. One approach requires site-specific and stable attachment of fluorophores to the components of translation machinery. Fluorescent tagging of the ribosome is a...More

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