Cloning and analysis of a novel cDNA from encoding a protein with an FYVE zinc finger domain

A cDNA library from Trichinella spiralis adults 3 days post-infection was screened with a cDNA probe, designated T54, derived from a newborn larvae subtracted cDNA library. Sequence analysis showed that the positive clone contained a cDNA insert of 1464bp in length with a single open reading frame of 1290bp, which encoded a protein of 429 amino acids with a putative molecular mass of 49.9kDa. Database analysis predicted the deduced protein had a leucine zipper motif and an FYVE zinc finger domain. The recombinant fusion protein was expressed and rabbit anti-recombinant protein sera reacted with a single peptide migrating at approximately 55kDa in crude worm extract from muscle larvae, adults and newborn larvae stages.