A Study on a Novel Biomimetic Antimicrobial Agent

Using biomimetic synthesis technology,we obtained a novel antimicrobial agent α-propargyl-γmethylene-γ- butyrolactone on the basis of protoanemonin. We characterized bacteriostatic effect and antimicrobial mechanism of the agent using physical and chemical analysis. We carried out the minimum inhibitory concentration (MIC) determination,suspension quantitative germicidal test,and other tests to verify its antimicrobial effect,the mechanism of which were primarily studied through cell wall integrity test and transmission electron microscopy. We found that the novel agent α-propargyl-γ-methylene-γ-butyrolactone had a good inhibitory effect on various bacterial species,especially on Malassezia globosa. Its MIC to malassezia was 0. 5mg/ml. Our suspension quantitative germicidal test,using Sodium thiosulfate as neutralizer,showed a high antibacterial efficiency that up to 99. 99% of Malassezia globosa is killed after exposed to 10mg/ml agent for only 1min. Membrane integrity experiments and transmission electron experiment suggested that the antimicrobial effect is not due to destroying the cell wall systerm,but it may have some impact on the cell wall.