Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air
Archives of Virology(2011)
摘要
Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×10 4 to 6.92×10 4 copies/m 3 air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.
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关键词
Newcastle Disease Virus,Avian Influenza Virus,Infectious Bronchitis Virus,Highly Pathogenic Avian Influenza,Infectious Bursal Disease Virus
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