Cholera toxin neutralization: a comparison of purified serum IgG and biliary secretory IgA antibodies

Rats were immunized three times with cholera toxin via the intraintestinal or intravenous route, and their respective biliary secretory IgA (sIgA) or serum IgG antibodies were affinity-purified on a cholera toxin immunoabsorbent. On a molar basis, the sIgA antibodies were roughly seven-fold more efficient than IgG antibodies in neutralizing cholera toxin in the ligated intestinal loop assay. Various explanations for this difference in neutralizing capacity are proposed.