Transverse fluctuation analysis of single extended DNA molecules
The European Physical Journal E(2003)
摘要
: We observed fluctuations of elongated DNA molecules by fluorescence microscopy. The molecules are fixed at both ends and undulate. Mode analysis of the thermally excited undulations of the labeled DNA molecules gives access to the spectral density of the amplitude fluctuations. From these measurements we estimate the tension acting on the DNA molecules. We found the forces to be within the entropic elasticity range of a typical DNA molecule (measured on the single-molecule level).
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关键词
PACS. 82.35.Pq Biopolymers,biopolymerization – 87.15.Ya Biomolecules: Fluctuations – 82.37.Rs Single molecule manipulation of proteins and other biological molecules
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