DNA extraction and fingerprinting of commercial rice cereal products

DNA was extracted from commercial rice cereal products using modified conventional methods (CTAB, SDS and a commercial kit) in large fragments (>3kb) and with relatively high yields (1.4–10.7μg DNA per g of sample) and was used as template for the amplification of a single copy rice gene (i.e. MIPS) fragment (ca. 850bp) and microsatellite DNAs (ca. 120–400bp). The cereal products were further discriminated by using six microsatellite markers. The usefulness of DNA analysis was discussed for quality control and authenticity testing of raw rice materials in rice-based food production, and to monitor genetically modified (GM) rice ingredients in commercial food products.